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KMID : 0369819960260020083
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Jorunal of Korean Pharmaceutical Sciences
1996 Volume.26 No. 2 p.83 ~ p.89
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Determination of DL-Carnitine Hydrochloride in Pharmaceutical Preparation by HPLC using UV Absorption Derivatives
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박준규/Park JK
신희종/김정우/Shin HJ/Kim JW
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Abstract
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A reverse-phase HPLC method to determine DL-Carnitine Hydrochloride in pharmaceutical preparation is described. UV absorption derivatives of DL-Carnitine Hydrochloride were formed with p-Bromophenacyl Bromide in an essentially quantitative manner using crown ether as catalyst. The DL-Carnitine-bromophenacyl ester absorbed UV radiation strongly at 254nm, allowing the detection of as small a quantity as 12.5ng of DL-Carnitine Hydrochloride. A linear defection range was 5×10?8∼5×10?7M of DL-Carnitine Hydrochloride. And the linear regression at various drug concentration was =0.999 (n=10). The DL-Carnitine Hydrochloride in pharmaceutical preparation was successfully derivatized and separated from other constituents by reverse phase HPLC with detection at 254 nm.
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KEYWORD
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DL-Carnitine Hydrochloride, HPLC, UV Absorption Derivative, P-Bromophen- acyl Bromide
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